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Fundamentals of Advanced Light Microscopy, August 2019

Time flies: it is already two years since the last "Fundamentals" lecture series. The Core Facility has many new users since then, and some of the others maybe also would appreciate a repetition of this presentation.

This is an extensive introduction or refresher course for users in the field of advanced light microscopy, including users of confocal microscopy. If you are a user of advanced microscopy and would like to gain a better insight of what you are actually doing, this is for you. This course will enable the participants to better understand the imaging process and thus to make better choices when selecting a microscope or microscope settings for their specific research question.

The course is geared toward scientists who are using or intend to use the Core Facility Bioimaging at the Biomedical Center (and therefore focuses on techniques available here), but also welcomes others who would like to expand or refresh their knowledge of the basics principles of modern light microscopic imaging.

No registration is necessary

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Time and Location

Monday, 05. August 2019, 14:00 - 17-30: Sessions 1 & 2
Tuesday, 06. August 2019, 14:00 - 17-30: Sessions 3 & 4

There will be a break between sessions.

Location: Monday, seminar room N.01.017, Tuesday, room N.02.017, both at the Biomedical Center's south wing (the part that faces the Mensa and the Biocenter).


  • Basic Microscope Optics for fluorescence microscopy (1)
  • Principles of Fluorescence and Fluorescence Microscopy (2)
  • Image processing and image presentation (2)
  • Basics of Digital Imaging (2)
  • Confocal Microscopy (point scanners) (3)
  • How to record a good image (3)
  • Deconvolution (3)
  • Multi-photon: fluorescence, SHG and THG (4)
  • Superresolution microscopy: STED and other approaches (4)
  • Fluorescence life time microscopy (FLIM) (4)

The number in brackets indicate the session for which the respective topic is planned. Most topics will build on the previous ones, so it is strongly recommended to join at the beginning.

Speaker: Steffen Dietzel


Below are download links for last time's presentations. A lot of copyrighted material was deleted, so that some slides look funny now. Also, last time FLIM was not included and is therefore missing in the files.

Download Part 1.                     Download Part 2.