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Fundamentals of Advanced Light Microscopy, November 2016

The "Fundamentals of Advanced Light Microscopy" is an event coorganized by the SFB 1064 Chromatin Dynamics, the SFB 914 Trafficking of Immune Cells in Inflammation, Development and Disease, and the Core Facility Bioimaging.

This is an extensive introduction or refresher course for advanced users in the field of light microscopy, including users of confocal microscopy. The course is geared toward scientists who are using or intend to use the Core Facility Bioimaging at the Biomedical Center, but also welcomes all others who would like to expand or refresh their knowledge of the basics principles of modern light microscopic imaging. This course will enable the participants to better understand the imaging process and thus to make better choices when selecting a microscope or microscope settings for their specific research question.

For registration please see below.

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Time and Location

Tuesday, 15. November 2016, 10:15-13:30: Session 1 & 2
Tuesday, 22. November 2016, 10:15-13:30: Session 3 & 4

There will be a coffee break between sessions.

Location: "Kleiner Hörsaal" at the Biomedical Center, Room N02.040.


  • Basic Microscope Optics for fluorescence microscopy (1)
  • Principles of Fluorescence and Fluorescence Microscopy (2)
  • Image processing and image presentation (2)
  • Basics of Digital Imaging (2)
  • Confocal Microscopy (point scanners) (3)
  • How to record a good image (3)
  • Deconvolution (3)
  • Multi-photon: fluorescence, SHG and THG (4)
  • Superresolution microscopy: STED and other approaches (4)

The number in brackets indicate the session for which the respective topic is planned. Most talks will build on the previous ones, so it is strongly recommended to join at the beginning.

Speaker: Steffen Dietzel


Registration is free. Please register here if you plan to come! This will allow us to plan the coffee breaks. Plus, we are curious from which institutions people are joining us. There is no registration deadline, but obviously early registrations are more helpful for our planning.