Malpighi 63x oil PSFs

This page contains PSF full width half measurements (FWHM) from Malpighi's 63x NA 1.4 oil immersion objective in reflection mode with gold beads. See this page for an explanation how these measurements were performed.

Two wavelengths, 488 nm and 638 nm were used, both at two pinhole sizes, 0.5 AU and open pinhole, resulting in four sets of measurements. Voxel size is 31.0 nm x 31.0 nm x 148 nm.

Theoretical FWHM sizes 

FWHM without pinhole or fully open pinhole in x,y according to 0.51*λ/1.4. FWHM with pinhole size zero in x,y according to 0.51*λ/(1.4*√2).

Wavlength	488 nm	638 nm
xy-FWHM with open Pinhole	179 nm	232 nm
xy-FWHM with Pinhole size 0	126 nm	164 nm

Plots of FWHM measurements

For details on these data, see tables below.

488nm, pinhole 05

488nm, pinhole max

638nm, pinhole 05

638nm, pinhole max

Explanation of table columns

Date: The date when the data set was recorded.

n: The number of beads evaluated in a given 3D image stacks.

FWHM max and FWHM min: A recorded PSF is usually not quite circular. Along the xy-plane, PSFj fits the PSF using a Gaussian ellipse. The largest and the smallest FWHM values found are given here in micrometer. The median value from the evaluated beads in the given data set is given.

FWHM z: The FWHM along the optical axis. Median value of the evaluated beads. All measurements were performed with PSFJ.

im xy: From each 3D image stack, the bead with the FWHMmin closest to the median was selected as example bead. The central section of the PSF is displayed in this image. Ideally, this image should reveal concentric rings of intensity values. To enlarge the image, adjust the magnification of your browser (probably with the ctrl+ keys, depending on your browser). Note that browsers may 'smoothen' images.

im xz: For the same bead, the xz-view. z-axis is from left to right, x-axis from top to bottom. Ideally this image should reveal a symmetric intensity distribution, with a mirror symmetry along the vertical and the horizontal axis. Reoccuring deviations, eg. a 'banana shape' would indicate a problem with the objective. Deviations for single beads may be caused by non-spherical beads or two or more very closely spaced beads.

im yz: For the same bead, the yz-view. See previous column for explanations. z-axis is from top to bottom, y-axis from left to right.

Filename: Filename of the original data set.

488nm, pinhole 05

Date	n=	FWHM max	FWHM min	FWHM z	im xy	im xz	im yz	Filename	notes
20170410	10	0.166	0.143	0.417				20170410_malpighi_63xoil_488_05_01.csv	01
20170410	17	0.17	0.146	0.429				20170410_malpighi_63xoil_488_05_02.csv	02
20170410	17	0.167	0.142	0.417				20170410_malpighi_63xoil_488_05_03.csv	03
20170516	7	0.169	0.145	0.37				20170516_malpighi_63xoil_488_05_01.csv	01
20170516	9	0.166	0.149	0.402				20170516_malpighi_63xoil_488_05_02.csv	02
20170516	14	0.179	0.145	0.413				20170516_malpighi_63xoil_488_05_03.csv	03
20170531	17	0.17	0.145	0.367				20170531_malpighi_63xoil_488_05_01.csv	01
20170531	18	0.168	0.146	0.383				20170531_malpighi_63xoil_488_05_02.csv	02
20170620	11	0.17	0.147	0.41				20170620_malpighi_63xoil_488_05_01.csv	01
20170620	6	0.166	0.144	0.373				20170620_malpighi_63xoil_488_05_02.csv	02
20170620	12	0.165	0.145	0.368				20170620_malpighi_63xoil_488_05_03.csv	03
20170717	14	0.161	0.139	0.422				20170717_malpighi_63xoil_488_05_oilfbetweenstacks.csv	oilfbetweenstacks
20170717	10	0.162	0.139	0.416				20170717_malpighi_63xoil_488_05_oilfbetweenstacks02.csv	oilfbetweenstacks02
20170717	7	0.166	0.147	0.388				20170717_malpighi_63xoil_488_05_oilfbetweenstacks03.csv	oilfbetweenstacks03
20170717	2	0.153	0.141	0.346				20170717_malpighi_63xoil_488_05_oilhfbetweenstacks.csv	oilhfbetweenstacks
20170717	4	0.173	0.143	0.361				20170717_malpighi_63xoil_488_05_oilhfbetweenstacks02.csv	oilhfbetweenstacks02
20170717	5	0.161	0.139	0.389				20170717_malpighi_63xoil_488_05_oilhfbetweenstacks03.csv	oilhfbetweenstacks03
20171218	3	0.248	0.163	0.58				20171218_malpighi_63xoil_488_05_01.csv	01
20171218	4	0.234	0.174	0.575				20171218_malpighi_63xoil_488_05_02.csv	02
20171218	6	0.242	0.173	0.53				20171218_malpighi_63xoil_488_05_03.csv	03
20180110	6	0.222	0.182	0.623				20180110_malpighi_63xoil_488_05_03.csv	03
20180110	5	0.236	0.182	0.633				20180110_malpighi_63xoil_488_05_04.csv	04
20180112	14	0.195	0.157	0.414				20180112_malpighi_63xoil_488_05_01nachRep.csv	01nachRep
20180124	2	0.178	0.149	0.412				20180124_malpighi_63xoil_488_05_01.csv	01
20180124	12	0.187	0.149	0.425				20180124_malpighi_63xoil_488_05_02.csv	02
20180124	13	0.19	0.149	0.422				20180124_malpighi_63xoil_488_05_03.csv	03
20180124	3	0.173	0.15	0.421				20180124_malpighi_63xoil_488_05_04.csv	04
20180124	8	0.187	0.147	0.425				20180124_malpighi_63xoil_488_05_05.csv	05
20180124	9	0.176	0.144	0.432				20180124_malpighi_63xoil_488_05_06.csv	06
20180124	5	0.183	0.146	0.454				20180124_malpighi_63xoil_488_05_07.csv	07
20180124	4	0.181	0.149	0.422				20180124_malpighi_63xoil_488_05_08.csv	08
20180124	4	0.185	0.147	0.472				20180124_malpighi_63xoil_488_05_09.csv	09
20180124	7	0.18	0.146	0.46				20180124_malpighi_63xoil_488_05_10.csv	10

488nm, pinhole max

Date	n=	FWHM max	FWHM min	FWHM z	im xy	im xz	im yz	Filename	notes
20170410	6	0.235	0.173	0.547				20170410_malpighi_63xoil_488_max_01.csv	01
20170410	15	0.232	0.179	0.552				20170410_malpighi_63xoil_488_max_02.csv	02
20170410	18	0.236	0.178	0.588				20170410_malpighi_63xoil_488_max_03.csv	03
20170516	7	0.243	0.172	0.512				20170516_malpighi_63xoil_488_max_.csv
20170516	8	0.24	0.173	0.523				20170516_malpighi_63xoil_488_max_02.csv	02
20170516	6	0.23	0.17	0.548				20170516_malpighi_63xoil_488_max_03.csv	03
20170531	13	0.239	0.173	0.486				20170531_malpighi_63xoil_488_max_01.csv	01
20170531	14	0.235	0.167	0.502				20170531_malpighi_63xoil_488_max_02.csv	02
20170531	4	0.233	0.171	0.49				20170531_malpighi_63xoil_488_max_03.csv	03
20170620	9	0.229	0.176	0.586				20170620_malpighi_63xoil_488_max_01.csv	01
20170620	4	0.231	0.174	0.462				20170620_malpighi_63xoil_488_max_02.csv	02
20170620	7	0.224	0.171	0.467				20170620_malpighi_63xoil_488_max_03.csv	03
20170717	6	0.234	0.172	0.532				20170717_malpighi_63xoil_488_max_oilfbetweenstacks.csv	oilfbetweenstacks
20170717	5	0.239	0.174	0.569				20170717_malpighi_63xoil_488_max_oilfbetweenstacks02.csv	oilfbetweenstacks02
20170717	5	0.241	0.168	0.543				20170717_malpighi_63xoil_488_max_oilfbetweenstacks03.csv	oilfbetweenstacks03
20170717	5	0.254	0.178	0.574				20170717_malpighi_63xoil_488_max_oilhfbetweenstacks.csv	oilhfbetweenstacks
20170717	4	0.246	0.181	0.544				20170717_malpighi_63xoil_488_max_oilhfbetweenstacks02.csv	oilhfbetweenstacks02
20170717	4	0.252	0.181	0.525				20170717_malpighi_63xoil_488_max_oilhfbetweenstacks03.csv	oilhfbetweenstacks03
20171218	20	0.248	0.167	0.586				20171218_malpighi_63xoil_488_max_01.csv	01
20171218	4	0.234	0.174	0.575				20171218_malpighi_63xoil_488_max_02.csv	02
20171218	14	0.241	0.169	0.562				20171218_malpighi_63xoil_488_max_03.csv	03
20180110	6	0.222	0.182	0.623				20180110_malpighi_63xoil_488_max_03.csv	03
20180110	5	0.236	0.182	0.633				20180110_malpighi_63xoil_488_max_04.csv	04
20180112	11	0.25	0.175	0.719				20180112_malpighi_63xoil_488_max_01nachRep.csv	01nachRep
20180124	9	0.249	0.175	0.673				20180124_malpighi_63xoil_488_max_02.csv	02
20180124	9	0.243	0.172	0.629				20180124_malpighi_63xoil_488_max_03.csv	03
20180124	1	0.231	0.172	0.674				20180124_malpighi_63xoil_488_max_04.csv	04
20180124	1	0.233	0.183	0.615				20180124_malpighi_63xoil_488_max_05.csv	05
20180124	1	0.235	0.185	0.705				20180124_malpighi_63xoil_488_max_06.csv	06
20180124	1	0.236	0.183	0.699				20180124_malpighi_63xoil_488_max_07.csv	07
20180124	1	0.224	0.178	0.651				20180124_malpighi_63xoil_488_max_08.csv	08
20180124	1	0.239	0.182	0.739				20180124_malpighi_63xoil_488_max_09.csv	09
20180124	2	0.242	0.175	0.75				20180124_malpighi_63xoil_488_max_10.csv	10

638nm, pinhole 05

Date	n=	FWHM max	FWHM min	FWHM z	im xy	im xz	im yz	Filename	notes
20170410	6	0.213	0.191	0.539				20170410_malpighi_63xoil_638_05_01.csv	01
20170410	6	0.215	0.191	0.541				20170410_malpighi_63xoil_638_05_02.csv	02
20170410	15	0.208	0.187	0.551				20170410_malpighi_63xoil_638_05_03.csv	03
20170516	4	0.209	0.196	0.51				20170516_malpighi_63xoil_638_05_01.csv	01
20170516	5	0.211	0.189	0.527				20170516_malpighi_63xoil_638_05_02.csv	02
20170516	6	0.207	0.188	0.544				20170516_malpighi_63xoil_638_05_03.csv	03
20170531	13	0.205	0.191	0.489				20170531_malpighi_63xoil_638_05_01.csv	01
20170531	10	0.21	0.189	0.525				20170531_malpighi_63xoil_638_05_02.csv	02
20170620	8	0.2	0.186	0.557				20170620_malpighi_63xoil_638_05_01.csv	01
20170620	5	0.204	0.183	0.507				20170620_malpighi_63xoil_638_05_02.csv	02
20170620	9	0.201	0.187	0.507				20170620_malpighi_63xoil_638_05_03.csv	03
20170717	8	0.211	0.187	0.58				20170717_malpighi_63xoil_638_05_oilfbetweenstacks.csv	oilfbetweenstacks
20170717	11	0.207	0.191	0.598				20170717_malpighi_63xoil_638_05_oilfbetweenstacks02.csv	oilfbetweenstacks02
20170717	6	0.206	0.186	0.529				20170717_malpighi_63xoil_638_05_oilfbetweenstacks03.csv	oilfbetweenstacks03
20170717	7	0.191	0.178	0.574				20170717_malpighi_63xoil_638_05_oilhfbetweenstacks.csv	oilhfbetweenstacks
20170717	10	0.201	0.181	0.583				20170717_malpighi_63xoil_638_05_oilhfbetweenstacks02.csv	oilhfbetweenstacks02
20170717	6	0.208	0.184	0.603				20170717_malpighi_63xoil_638_05_oilhfbetweenstacks03.csv	oilhfbetweenstacks03
20171218	17	0.247	0.166	0.605				20171218_malpighi_63xoil_638_05_01.csv	01
20171218	4	0.241	0.171	0.577				20171218_malpighi_63xoil_638_05_02.csv	02
20171218	6	0.241	0.175	0.566				20171218_malpighi_63xoil_638_05_03.csv	03
20180110	4	0.221	0.18	0.623				20180110_malpighi_63xoil_638_05_03.csv	03
20180110	7	0.237	0.182	0.636				20180110_malpighi_63xoil_638_05_04.csv	04
20180112	12	0.226	0.192	0.589				20180112_malpighi_63xoil_638_05_01nachRep.csv	01nachRep
20180124	2	0.211	0.191	0.541				20180124_malpighi_63xoil_638_05_01.csv	01
20180124	6	0.227	0.195	0.537				20180124_malpighi_63xoil_638_05_02.csv	02
20180124	11	0.219	0.194	0.538				20180124_malpighi_63xoil_638_05_03.csv	03
20180124	1	0.214	0.191	0.547				20180124_malpighi_63xoil_638_05_04.csv	04
20180124	5	0.207	0.19	0.584				20180124_malpighi_63xoil_638_05_05.csv	05
20180124	2	0.212	0.192	0.559				20180124_malpighi_63xoil_638_05_06.csv	06
20180124	4	0.216	0.189	0.579				20180124_malpighi_63xoil_638_05_07.csv	07
20180124	2	0.213	0.195	0.613				20180124_malpighi_63xoil_638_05_08.csv	08
20180124	2	0.204	0.188	0.612				20180124_malpighi_63xoil_638_05_09.csv	09
20180124	4	0.203	0.189	0.603				20180124_malpighi_63xoil_638_05_10.csv	10

638nm, pinhole max

Date	n=	FWHM max	FWHM min	FWHM z	im xy	im xz	im yz	Filename	notes
20170410	7	0.281	0.229	0.682				20170410_malpighi_63xoil_638_max_01.csv	01
20170410	4	0.27	0.231	0.679				20170410_malpighi_63xoil_638_max_02.csv	02
20170410	12	0.278	0.234	0.688				20170410_malpighi_63xoil_638_max_03.csv	03
20170516	4	0.286	0.226	0.662				20170516_malpighi_63xoil_638_max_01.csv	01
20170516	6	0.285	0.225	0.667				20170516_malpighi_63xoil_638_max_02.csv	02
20170516	5	0.271	0.227	0.683				20170516_malpighi_63xoil_638_max_03.csv	03
20170531	10	0.266	0.224	0.624				20170531_malpighi_63xoil_638_max_01.csv	01
20170531	10	0.278	0.222	0.655				20170531_malpighi_63xoil_638_max_02.csv	02
20170620	6	0.277	0.23	0.682				20170620_malpighi_63xoil_638_max_01.csv	01
20170620	4	0.281	0.231	0.6				20170620_malpighi_63xoil_638_max_02.csv	02
20170620	6	0.279	0.225	0.625				20170620_malpighi_63xoil_638_max_03.csv	03
20170717	5	0.26	0.221	0.692				20170717_malpighi_63xoil_638_max_oilfbetweenstacks.csv	oilfbetweenstacks
20170717	6	0.267	0.22	0.74				20170717_malpighi_63xoil_638_max_oilfbetweenstacks02.csv	oilfbetweenstacks02
20170717	3	0.275	0.22	0.689				20170717_malpighi_63xoil_638_max_oilfbetweenstacks03.csv	oilfbetweenstacks03
20170717	3	0.257	0.225	0.668				20170717_malpighi_63xoil_638_max_oilhfbetweenstacks.csv	oilhfbetweenstacks
20170717	7	0.251	0.226	0.68				20170717_malpighi_63xoil_638_max_oilhfbetweenstacks02.csv	oilhfbetweenstacks02
20170717	5	0.256	0.223	0.683				20170717_malpighi_63xoil_638_max_oilhfbetweenstacks03.csv	oilhfbetweenstacks03
20171218	2	0.239	0.167	1.095				20171218_malpighi_63xoil_638_max_01.csv	01
20171218	4	0.241	0.171	0.577				20171218_malpighi_63xoil_638_max_02.csv	02
20171218	6	0.241	0.175	0.566				20171218_malpighi_63xoil_638_max_03.csv	03
20180110	8	0.222	0.182	0.614				20180110_malpighi_63xoil_638_max_03.csv	03
20180110	7	0.237	0.181	0.633				20180110_malpighi_63xoil_638_max_04.csv	04
20180112	6	0.291	0.23	0.718				20180112_malpighi_63xoil_638_max_01nachRep.csv	01nachRep
20180124	2	0.293	0.24	0.685				20180124_malpighi_63xoil_638_max_01.csv	01
20180124	4	0.271	0.223	0.693				20180124_malpighi_63xoil_638_max_02.csv	02
20180124	7	0.273	0.226	0.669				20180124_malpighi_63xoil_638_max_03.csv	03
20180124	1	0.269	0.238	0.662				20180124_malpighi_63xoil_638_max_04.csv	04
20180124	4	0.278	0.226	0.69				20180124_malpighi_63xoil_638_max_05.csv	05
20180124	1	0.265	0.229	0.68				20180124_malpighi_63xoil_638_max_06.csv	06
20180124	5	0.273	0.235	0.681				20180124_malpighi_63xoil_638_max_07.csv	07
20180124	1	0.267	0.225	0.716				20180124_malpighi_63xoil_638_max_08.csv	08
20180124	4	0.267	0.234	0.699				20180124_malpighi_63xoil_638_max_09.csv	09
20180124	1	0.255	0.224	0.714				20180124_malpighi_63xoil_638_max_10.csv	10