Program

Talks

A “BMC trailblazer lecture” by Jennifer Lippincott-Schwartz will be given on Monday, 11:00. This lecture is not part of the workshop, it just happens to be on the same day in the same lecture hall, two hours before our workshop starts. In her lab, photoactivation localization microscopy (PALM) was developed, one of the first superresolution microscopy techniques. I imagine that some scientists who are interested in FLIM might also be interested in what she has to say, therefore I put this information here.

Monday, 26. Feb. 2024

	Title	Speaker
Getting started:
13:15	Welcome
13:30-14:15	Introduction to FLIM	Stefanie Weidkamp-Peters, Düsseldorf
14:15-14:45	Tension sensors: FLIM measurement of Flipper-TR in live cells	Juan Manuel Garcia Arcos, Geneva
14:45-15:15	Break
Label-free metabolic FLIM:
15:15-15:45	Label-free metabolic imaging of living tissues by Fluorescence Lifetime Microscopy of endogenous biomarkers	Chiara Stringari, Paris
15:45-16:15	Metabolic FLIM (NADPH) and oxygen dependent PLIM	Angelika Rück, Ulm
16:15-16:45	Label free wide-field metabolic imaging of NADH and FAD	André Weber, Magdeburg
16:45-17:15	Break
Advanced applications:
17:15-17:45	Investigating HIV Maturation and Nanoparticle Stability with phasor FLIM	Don Lamb, Munich
17:45-18:15	Video rate volumetric FLIM with nanosecond pixel dwell time	Sebastian Karpf, Lübeck
18:15-18:45	Separation of fluorochrome in the same spectral channel with FLIM in confocal and STED microscopy	Mariano Gonzalez Pisfill, Munich

Tuesday, 27. Feb 2024

	Title	Speaker
FLIM-FRET and tension sensors
9:00-9:30	FLIM-FRET data acquisition and analysis	Ali Gheisari, Munich
9:30-10:00	Recording dimerization and ligand binding by confocal FLIM-FRET with GPCRs in native lipid environments of cells, membrane vesicles and polymer-derived lipid nanodiscs	Michael Börsch, Jena
10:00-10:30	Characterizing the system: FLIM standards	Michael Börsch, Jena
10:30-11:00	FLIM standards – panel discussion	various
11:00-11:30	Break
11:30-12:00	Imaging live-cell biochemistry by light	Alessandro Esposito, London
12:00-12:30	Short talks on community developed FLIM-software: FlimJ; FlimFit; FLUTE; napari-FLIM-phasor-plotter	Sebastian Karpf, Anca Margineanu, Chiara Stringari, Cornelia Wetzker

Parallel hands-on sessions

There are six slots for hands-on sessions, each 2h30min, scheduled as listed below. Thus each participant could join up to six hands-on sessions, if enough spaces are available. Please note that while we will make an effort to accommodate every participant's preferences, access to any particular topic cannot be guaranteed. Accepted participants will have the opportunity to communicate their preferred choices. Typically, up to seven participants will be accepted for each individual session. We aim to conduct hands-on sessions on each topic several times to allow more workshop participants to join.

Tuesday, 27. Feb. 2024

13:30-16:00 Hands on 1

16:30-19:00 Hands on 2

Wednesday, 28. Feb. 2024

9:30-12:00 Hands on 3

13:00-15:30 Hands on 4

16:00-18:30 Hands on 5

Thursday, 29. Feb. 2024

9:30-12:00 Hands on 6

Hands-on session topics

Download workshop booklet with hands-on session abstracts!

Download list showing which topics are still available (will be updated frequently)

For each session topic, find below designation, title, conducting persons and the hardware on which it will be performed. At least two hands-on sessions will be conducted for each topic.

N11-1: Analyzing data with FLIMfit
Anca Margineanu, Max-Delbrück-Center, Berlin
Participants Notebooks

N11-2: How to compute fluorescence lifetime photon-by-photon using a cuvette-based optical system
Alessandro Rossetta, FLIM LABS S.r.l.
FLIM LABS equipment

N13-1 Metabolic FLIM (NADPH) and oxygen dependent PLIM
Angelika Rück, Daniela dos Santos, Universität Ulm
Metabolic Imager, Becker & Hickl with Toptica 780 nm and 920 nm femtosecond laser and ibidi stage-top incubator

N13-2 FLIM-FRET with fluorescent proteins
Michael Börsch & Lukas Spantzel, Jena
Metabolic Imager, Becker & Hickl with Toptica 780 nm and 920 nm femtosecond laser and ibidi stage-top incubator

N14-1 How to measure FLIM, PLIM and both simultaneously
Yuansheng Sun, ISS
Q2 confocal with pulsed lasers (375, 488, 635nm) and 2 detectors (ISS Hybrid & Excelitas SPAD), ISS

N14-2 How to use the phasor plots to separate two or three species
Yuansheng Sun, ISS
Q2 confocal with pulsed lasers (375, 488, 635nm) and 2 detectors (ISS Hybrid & Excelitas SPAD), ISS

N14-3 How to use the phasor plots to process and analyze FLIM-FRET data
Yuansheng Sun, ISS
Q2 confocal with pulsed lasers (375, 488, 635nm) and 2 detectors (ISS Hybrid & Excelitas SPAD), ISS

N15-1 Introduction to Frequency Domain FLIM and FRET
Jeroen Wehmeijer, Lambert Instruments
Lambert Instruments LIFA vTAU camera-based FLIM system

N16-1 Video-rate volumetric FLIM with Nanosecond pixel dwell time (SLIDE)
Florian Sommer & Stefan Meyer, Medizinisches Laserzentrum Lübeck GmbH
SLIDE System, Medizinisches Laserzentrum Lübeck GmbH

N19-1 Widefield FLIM - Frequency Domain Fluorescence Lifetime Imaging for Widefield Microscopy
Gerhard Holst, PCO
pco.flim PRO camera w. Nikon stand, 445nm or 488nm or 636nm exc.

N20-1 FLIM-FRET measurements in plant tissue
Stefanie Weidtkamp-Peters, Universität Düsseldorf
Nikon AX with PicoQuant FLIM kit, PicoQuant

N20-2 Studying cell signaling in single living cells
Alessandro Esposito, Brunel University, London
Nikon AX with PicoQuant FLIM kit, PicoQuant

N21-1 Label free wide-field metabolic imaging of NADH and FAD
André Weber, Leibniz-Institut für Neurobiologie, Magdeburg
Lincam with Nikon stand and Omikron light enginge, Photonscore

CF43-1 FLIM measurement of Flipper-TR in live cells
Juan Manuel García-Arcos, Université de Genève
Leica SP8 STED FALCON, Core Facility Bioimaging

CF43-2 Separation of fluorochromes in the same spectral channel with FLIM in confocal and STED microscopy
Mariano Gonzalez Pisfill, Ludwig-Maximilians-Universität München
Leica SP8 STED FALCON, Core Facility Bioimaging

CF44-1 Label-free metabolic FLIM with 2 photon excitation
Chiara Stringari, Institut Polytechnique de Paris
SP8 MP WLL DM8 FALCON, Core Facility Bioimaging

CF44-2 Environmental sensing via lifetime: From TauSense to FALCON
Heike Glauner & Daniel Smeets, Leica Microsystems
Stellaris 8 FALCON, Leica Microsystems

CuP1: Fluorescence lifetime imaging of viral particles and nanocarriers using the phasor approach
Irene Gialdini, Ludwig-Maximilians-Universität München
Self-constructed system, Don Lamb group, Dept. Chemistry and Pharmacy (5 min walk)

CuP2: Structural Biology with a FLIM Microscope Using Graphene Near Field Quenching
Tim Schröder & Giovanni Ferrari
Self-constructed system, Philip Tinnefeld group, Dept. Chemistry and Pharmacy (5 min walk)

MPI1: Lifetime-based Imaging of Order Heterogeneity in in vitro membrane systems
Jan Hagen Krohn & Yusuf Qutbuddin, Max-Planck-Institut für Biochemie
PicoQuant MicroTime 200 system, Schwille lab, Max-Planck-Institut für Biochemie (10 min walk)

MPI2: Sub-resolution spatial information from FLIM
Martin Spitaler & Markus Oster, Max-Planck-Institut für Biochemie
Leica SP8 FALCON, Imaging Facility, Max-Planck-Institut für Biochemie (10 min walk)