This page contains measurements of PSF full width half maxima.
For details on these data, see tables below.
Date: The date when the data set was recorded.
n: The number of beads evaluated in a given 3D image stacks.
FWHM max and FWHM min: A recorded PSF is usually not quite circular. Along the xy-plane, PSFj fits the PSF using a Gaussian ellipse. The largest and the smallest FWHM values found are given here in micrometer. The median value from the evaluated beads in the given data set is given.
FWHM z: The FWHM along the optical axis. Median value of the evaluated beads. All measurements were performed with PSFJ.
im xy: From each 3D image stack, the bead with the FWHMmin closest to the median was selected as example bead. The central section of the PSF is displayed in this image. Ideally, this image should reveal concentric rings of intensity values. To enlarge the image, adjust the magnification of your browser (probably with the ctrl+ keys, depending on your browser). Note that browsers may 'smoothen' images.
im xz: For the same bead, the xz-view. z-axis is from left to right, x-axis from top to bottom. Ideally this image should reveal a symmetric intensity distribution, with a mirror symmetry along the vertical and the horizontal axis. Reoccuring deviations, eg. a 'banana shape' would indicate a problem with the objective. Deviations for single beads may be caused by non-spherical beads or two or more very closely spaced beads.
im yz: For the same bead, the yz-view. See previous column for explanations. z-axis is from top to bottom, y-axis from left to right.
Filename: Filename of the original data set.
| Date | n= | FWHM max | FWHM min | FWHM z | im xy | im xz | im yz | Filename | notes |
|---|---|---|---|---|---|---|---|---|---|
| 20170410 | 10 | 0.166 | 0.143 | 0.417 |  |
 |
 |
20170410_malpighi_63xoil_488_05_01.csv | 01 |
| 20170516 | 7 | 0.169 | 0.145 | 0.37 |  |
 |
 |
20170516_malpighi_63xoil_488_05_01.csv | 01 |
| 20180110 | 6 | 0.222 | 0.182 | 0.623 |  |
 |
 |
20180110_malpighi_63xoil_488_05_03.csv | 03 |
| Date | n= | FWHM max | FWHM min | FWHM z | im xy | im xz | im yz | Filename | notes |
|---|---|---|---|---|---|---|---|---|---|
| 20170410 | 6 | 0.235 | 0.173 | 0.547 |  |
 |
 |
20170410_malpighi_63xoil_488_max_01.csv | 01 |
| 20170516 | 8 | 0.24 | 0.173 | 0.523 |  |
 |
 |
20170516_malpighi_63xoil_488_max_02.csv | 02 |
| 20180110 | 6 | 0.222 | 0.182 | 0.623 |  |
 |
 |
20180110_malpighi_63xoil_488_max_03.csv | 03 |
| Date | n= | FWHM max | FWHM min | FWHM z | im xy | im xz | im yz | Filename | notes |
|---|---|---|---|---|---|---|---|---|---|
| 20170410 | 6 | 0.213 | 0.191 | 0.539 |  |
 |
 |
20170410_malpighi_63xoil_638_05_01.csv | 01 |
| 20170516 | 4 | 0.209 | 0.196 | 0.51 |  |
 |
 |
20170516_malpighi_63xoil_638_05_01.csv | 01 |
| 20180110 | 4 | 0.221 | 0.18 | 0.623 |  |
 |
 |
20180110_malpighi_63xoil_638_05_03.csv | 03 |
| Date | n= | FWHM max | FWHM min | FWHM z | im xy | im xz | im yz | Filename | notes |
|---|---|---|---|---|---|---|---|---|---|
| 20170410 | 7 | 0.281 | 0.229 | 0.682 |  |
 |
 |
20170410_malpighi_63xoil_638_max_01.csv | 01 |
| 20170516 | 4 | 0.286 | 0.226 | 0.662 |  |
 |
 |
20170516_malpighi_63xoil_638_max_01.csv | 01 |
| 20180110 | 8 | 0.222 | 0.182 | 0.614 |  |
 |
 |
20180110_malpighi_63xoil_638_max_03.csv | 03 |